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WHOLE SPORES
COLOR: Bright
yellowish orange (0-10-60-0) to dark brownish orange (0-40-100-0),
with many bright orange (0-20-100-0)
SHAPE:
Globose, subglobose, occasionally ovoid
SIZE DISTRIBUTION:
100-240 µm, mean = 171 µm, n = 110
SUBCELLULAR STRUCTURE OF SPORES
SPORE WALL: Consisting of two layers (L1 and L2) that differentiate consecutively as spores develop.
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| In
PVLG |
In PVLG & Melzer's reagent |
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L1: A semiflexible, hyaline layer, 1-2 µm thick; usually sloughing and therefore absent in field-collected specimens and many spores in a mature pot culture..
L2:
A rigid layer consisting of fine adherent sublayers (or laminae), yellow to
orange in color, 5-14 µm thick. Evenly distributed warts, 0.8-1.7 µm high in
cross view, 0.5-0.7 µm diam in plan view, are reported by Blaszkowski and Tadych
(1997) as projecting inward from the innermost sublayer, but these are seen
only in a small percentage of spores in this reference culture. Given that variability,
this trait is not a taxonomically informative character.
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SHAPE:
Cylindrical to somewhate
flared, occasionally recurved.
WIDTH: 15-28
µm (mean = 21 µm).
COMPOSITE WALL THICKNESS:
4.5-7 µm at spore base.
WALL
STRUCTURE: Two
layers (L1 and L2) continuous with the inner two layers of the spore wall and
with the same properties. L2 extends for only 20-25 µm down length of hypha.
OCCLUSION:
Recurved septum from the innermost sublayer of L2 of the spore wall.
Not observed.
Not observed.
NOTES
Color of the spores is quite distinctive among species of Glomus described thus far. The size range of spores in the reference culture is considerably extended at the small end than is published in the protologue (the lower limit reported to be 145 µm).
REFERENCES
Blaszkowski, J. and M.Tadych.
1997. Glomus multiforum and G. verruculosum, two new species from
Poland. Mycologia 89:804-811.