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Glomus spurcum -- preparing clean healthy spores for shipment
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Extracted spores. This photo shows a random population of spores collected manually after sucrose centrifugation and washing. Because spores of G. spurcum are colorless, departures from the healthy condition are fairly easily detected. | |
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Degraded or dead spores. Small colorless or light-colored spored species like G. spurcum show infrequent parasitism. More often, spores are dead and are devoid of contents (as seen at left). These spore husks do not degrade rapidly and can persist in continuous cultures for generations. To eliminate this background "noise", new cultures need to be started from spores after 2-3 culture generations. |
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Healthy spores. Glomus spurcum spores often have transparent centers because of lipid coalescence. They also may resemble other hyaline Glomus spp. with a fairly homogeneous distribution of smaller lipid globules. Either phenotype indicates good health and stability in this feature afer 48 hr in a refrigerator indicates a population ready for shipment. |
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